Urine is an important source of iPSC generation. Creative Bioarray has established a proven experimental system to help researchers generate human iPSCs from urine-derived renal tubular cells. We have the confidence to provide our clients with professional and efficient scientific services to make drug discovery and kidney research possible.
Renal Tubular Cells Reprogramming
The human urinary system consists of a variety of small tube networks, which provides a relatively large total surface area in comparison to the skin. On a daily basis, a large number of cells are shed from the human urinary system, including the urethra and ureters. In addition, urine production is a key physiological process for normal internal homeostasis in humans. It has been reported that urine-derived iPSCs can differentiate into a variety of cells with significant advantages, including urinary epithelial cells, neural cells, endothelial cells, osteoblasts, skeletal myogenic cells, adipocytes, and chondrocytes.
In 2011, scientists have obtained urine-derived iPSC (UiPSC) by reprogramming cells in human urine, and the approach of reprogramming urine cells has evolved from integration to non-integration. The development of reprogramming techniques for urine cells has immeasurable potential value for regenerative medicine. Researchers have obtained stable and well-differentiated induced pluripotent stem cells by reprogramming renal tubular cells derived from human urine, suggesting that they are one of the ideal sources of induced pluripotent stem cells.
Fig.1 Schematic diagram of the renal tubular cell reprogramming procedure. (Chen, 2015)
Our Services
Researchers at Creative Bioarray have worked to develop strategies for rapid, reproducible, and efficient generation of iPSC from renal tubular epithelial cells. The non-integrative approach we have established contributes well to genetic stability compared to that observed by conventional retroviral transduction. However, the reprogramming process leads to uncontrollable stochastic effects that may interfere with drug discovery and disease modeling research. In order to generate "safer, higher quality" iPSCs for transplantation and disease modeling, we offer the following services to help our clients mitigate these issues.
- Reducing the number of factors used for reprogramming
- Engineering more efficient transcription factors
- Combining chemical compounds with different reprogramming factors
- Developing non-integration methods
Applications
- Development of therapies for kidney related diseases
- Establishment of reliable drug-testing platforms
- Disease modeling in vitro
Advantages of Our Services
- Simple, reproducible, noninvasive
- Efficient and cost-effective
- Safer and higher quality
Over the years, Creative Bioarray has focused on providing professional and efficient cell reprogramming services to our clients. Our established methods for generating iPSC from renal tubular cells present in urine provide a reliable cell platform for researchers to develop kidney differentiation protocols. If you need our technical support, please feel free to contact us.
References
- Chen, W.; et al. Generation of induced pluripotent stem cells from renal tubular cells of a patient with Alport syndrome. International journal of nephrology and renovascular disease. 2015, 8: 101.
- Zhou, T.; et al. Generation of induced pluripotent stem cells from urine. Journal of the American Society of Nephrology. 2011, 22(7): 1221-1228.