The selection of effective and safe delivery systems is critical to achieving successful cell reprogramming with specific reprogramming factors. Creative Bioarray is focusing on helping customers develop physical delivery systems to improve the efficiency and safety of reprogramming. Our advanced reprogramming platform and specialized scientific services will accelerate your research in the field of cell reprogramming.
Background
Large advances have been made in the field of cell reprogramming including iPSC reprogramming and the subsequent development of direct cell reprogramming. Viral vectors have been used as the vector of choice during cell reprogramming because of their high transduction efficiency. However, the unpredictable gene dysfunction, limited DNA packaging capacity, immunogenicity and tumorigenicity of viral gene transmission limit the clinical application of nuclear reprogramming techniques. There is a clear need in the field for new technologies capable of delivering reprogramming factors in a safe and efficient manner for successful translation from laboratory work products to the clinic.
For many years, researchers at Creative Bioarray have focused on developing non-integrative, non-viral methods to introduce transgenes into cells. With extensive experience and expertise in the field of cell reprogramming, we are well-positioned to help our clients develop physical delivery systems for direct delivery of reprogramming factors into the nucleus or cytoplasm by membrane disruption methods, involving electroporation and mechanical disruption.
Fig.1 Microchannel or microfluidic electroporation for cargo delivery. (Fang, 2020)
Physical Delivery Systems
- Development of Electroporation Techniques for Cell Reprogramming
- Development of Mechanical Disruption Techniques for Cell Reprogramming
Since it was first reported, electroporation has received much attention as one of the most popular non-viral gene delivery techniques. In order to efficiently transfect cells and avoid excessive cell damage caused by high electric fields, we have developed several electroporation techniques for cell reprogramming.
Mechanical destruction is a method of destroying cell membranes for transient transmission. Combined with the progress in the field of microfluidics and nanotechnology, our researchers help customers develop mechanical destruction methods and apply them to the field of cell reprogramming.
Advantages
- No mutation, no immunogenicity, low or no genetic integration
- Deliver diversified cargos without size restrictions
- Rapid and transient transfection
Why choose us?
As a professional cell reprogramming service platform, Creative Bioarray has the most specialized technology and means to help our customers improve the efficiency of cell reprogramming by developing physical delivery systems.
- We have the flexibility to modify the solutions according to the scientific needs of customers.
- Our company covers a wide range of cell characterization services to ensure that the physical delivery systems developed are efficient and safe.
Creative Bioarray specializes in the field of cellular reprogramming and has made significant progress. We help our customers improve the efficiency and quality of cell reprogramming by combining micro/ nanotechnologies. As a cell reprogramming technology platform service company with advanced technology, we can provide you with a variety of solutions to meet your specific needs. If you are interested, please contact us directly.
Reference
- Fang, J.; et al. Engineering biomaterials with micro/nanotechnologies for cell reprogramming. ACS nano. 2020, 14(2): 1296-1318.