Creative Bioarray is committed to developing a variety of methods to improve the reproducibility of human iPSC derivation, growth and differentiation to support our customers' research projects. Our extensive experience and knowledge in the field of cell reprogramming allow us to design experimental protocols according to the specific project requirements of our clients and provide high-quality scientific services.
Introduction
iPSCs have been highly successful in human development research, human disease research, tissue regeneration and repair, and the development of new therapies. Although various methods to enable more efficient derivation, growth, and differentiation of iPSCs are currently being investigated, there remains significant variability in genomic integrity, reprogramming efficiency, and differentiation potential of iPSCs derived from individual fibroblasts or tissue samples. These barriers limit the realization of the full potential of human iPSC in research and clinical practice.
A variety of factors influence the derivation of iPSCs and their stability and differentiation, including the features of the starting cell or tissue sample (e.g., donor age, anatomical location and tissue type, physiological and disease status), the selection of growth factors and other culture conditions, the approach used to induce pluripotency (e.g., TFs, cell fusion and small molecules), and the method of storage of cell lines. The ability to grow and maintain sufficient numbers of iPSC lines in culture media without altering their properties and to identify and authenticate iPSC lines are further challenges for investigators to face.
Fig.1 Balancing serendipity and reproducibility in the experimental system for nervous system diseases study. (Anderson, 2021)
Strategies
Our researchers have developed several strategies to improve the reproducibility of iPSC reprogramming.
- Validate the starting cell population using a set of standardized markers for phenotypic analysis.
- Use a more efficient and standardized method to measure iPSCs potency.
- Use multiple methods to confirm iPSCs pluripotency to improve the reproducibility in iPSCs differentiation protocols.
- Optimize cell growth and differentiation conditions using small molecules.
- Standardize reagents and protocols. Using high-quality reagents with reliable performance is critical to establishing consistent iPSCs expansion and differentiation protocols.
Advantages
- Experienced and professionally educated lab technicians
- Advanced cell reprogramming technology and standardized experimental protocols
- Efficient service model
- Strict experimental control system and reliable services
Creative Bioarray has years of experience in customizing and optimizing experimental conditions, and our professional technology platform will help you improve the reproducibility of iPSC reprogramming through a variety of strategies. If you need technical support, please contact us and we will provide you with a considerate one-stop service and detailed scientific experimental reports.
Reference
- Anderson, N.C.; et al. Balancing serendipity and reproducibility: Pluripotent stem cells as experimental systems for intellectual and developmental disorders. Stem cell reports. 2021, 16(6): 1446-1457.