The genetic integrity of iPSCs is critical to their effectiveness as disease models and potential therapeutic use.Creative Bioarray is committed to providing high-throughput genomic sequencing analysis for iPSCs to meet the specific needs of our clients in iPSC characterization.
Background
iPSC lines contain deleterious genetic mutations, which will greatly weaken the biomedical utility of iPSCs. Although iPSCs are functionally similar to ESCs, recent microarray studies have demonstrated that human iPSCs carry higher levels of DNA copy number variation than ESCs. The main reasons for this result include: (1) reprogramming involves the expression of known oncogenes and is enhanced by downregulating genes that promote genomic stability; (2) reprogramming involves global epigenetic remodeling, which may have mutagenicity or result in the activation of endogenous retroelements; (3) iPSC comes from differentiated cell types rather than early embryos, indicating that somatic mutations in donor cells may bring genetic diversity to these cell lines, which may be harmful.
A variety of experimental protocols for generating iPSC lines have been established. However, to avoid the risk of cancer or dysfunction caused by some genetic variation when using these cells for regenerative therapy intervention, the detailed characterization of genetic differences between donor and derived cells is still an area that needs to be actively developed to ensure validity and safety.
Fig.1 Detection of sequence variations in iPSCs and fibroblast subclones from the same fibroblast population. (Kwon, 2017)
Genome Sequencing Analysis Services
Creative Bioarray has developed several efficient strategies to provide high-quality iPSC genomic sequencing services to our clients. we provide technical support including but not limited to:
- Whole-genome sequencing
- Single nucleotide polymorphism (SNP) arrays
- cDNA expression analysis
We use whole-genome DNA sequencing and comprehensive structural variation (SV) detection algorithms to help our clients monitor the mutational burden of iPSCs.
We provide high-resolution SNP arrays for searching de novo copy number variations (CNVs) in iPSC clones and validate the detected CNVs using secondary approaches.
Applications
- Establishment of a suitable pipeline for iPSCs
- Genetic evaluation of each individual iPSC clone prior to its use for therapeutic purposes
- Research on cellular mechanisms
Advantages
- Advanced gene sequencing equipment
- High-resolution diagnostic methods
- High-speed and high-throughput
- Low-cost and high-efficiency
Creative Bioarray provides high-quality and high-throughput genome sequencing services to help our clients assess the genetic integrity of iPSCs. Our comprehensive iPSC characterization services can help you understand a wide range of iPSC characteristics quickly and efficiently at a competitive price. If you need our scientific services, please contact us for more details.
Reference
- Kwon, E.M.; et al. iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations. Proceedings of the National Academy of Sciences. 2017, 114(8): 1964-1969.