As an important farm animal, pigs are considered to be one of the best candidates for human disease modeling and donor xenotransplantation. Creative Bioarray has made some progress in the establishment of porcine iPSC lines, and we perform a variety of cell characterization services to ensure high-quality porcine iPSCs. Our efficient and professional scientific services will accelerate your research in the field of iPSC reprogramming of farm animals.
Porcine iPSCs
Human iPSCs have shown great therapeutic promise in the field of regenerative medicine. Therefore, it is necessary to test the effectiveness and safety of iPSCs-based therapies using appropriate animal models before translating them into the clinic. Rodents have been widely used in preclinical trials, but they can not accurately reflect human conditions because of significant differences in physiology and development. In many cases, pigs are considered to be the superior choice over rodent models. Pigs are more similar to humans in terms of genome, organ size, anatomical, immunological and physiological characteristics. In addition, porcine models are cost-effective because they are a by-product of slaughterhouses, so they can be widely used compared with other large animal models.
Notably, with the advancement of interspecies blastomere complementation, human organs produced in pigs have good prospects in addressing the worldwide shortage of organs for transplantation. Chimeric-competent porcine iPSCs could serve as a homozygous control and provide valuable information about the molecular and functional characteristics of the derived organs. However, current research on porcine and other large animal iPSCs is fraught with many challenges. Developing strategies for efficient generation of porcine iPSCs and improving the quality of porcine iPSCs are important for future preclinical studies based on porcine iPSCs.
Fig.1 Porcine embryonic stem cells have great significance and potential for wide application. (Han, 2019)
Our Services
With a dedicated and innovative research team, we have generated porcine iPSCs using a variety of induction methods and reprogrammed cells have been proved to show a certain degree of pluripotency. We will provide our customers with a one-stop service to help them obtain high-quality porcine iPSCs.
- We generated porcine iPSCs by infecting porcine pericytes or embryonic fibroblasts with retroviral vectors encoding reprogramming factors and subsequently cultured in a modified medium.
- We offer a variety of cell characterization services including examination of the expression of pluripotency-related TFs and surface markers, transcriptome profiling, and in vitro and in vivo differentiation capabilities to assess the pluripotency of reprogrammed iPSCs.
Applications
- Modeling of embryonic development and disease processes
- Efficacy and safety evaluation of stem cell related therapies
- Study on transplantation medicine, immunology and circulatory
Advantages
- Advanced cell reprogramming technology and extensive successful experience
- High-quality service and reliable technical support
- Fast turnaround time
As a company specializing in cell reprogramming services, Creative Bioarray helps our clients develop efficient and safe reprogramming strategies and improve culture conditions to establish stable porcine iPSCs. Our expert technical support will accelerate biological research on this species and enhance its utility as an animal model. If you need further details, please contact us.
Reference
- Han, J.; et al. Porcine pluripotent stem cells: progress, challenges and prospects. Frontiers of Agricultural Science and Engineering. 2019, 6(1): 8-27.