Equine iPSCs not only hold promise for the veterinary field, but also offer the opportunity to validate stem cell-based therapies using horses before entering the human clinical setting. Creative Bioarray is committed to providing efficient technical support to help clients reprogram various equine somatic cells into iPSCs and examine their pluripotency characteristics. Our specialized scientific services will help you explore the therapeutic potential of equine iPSCs for muscle injury.
Equine iPSCs
The domesticated horse is of great value in sports and recreational fields and has great potential as an excellent model for certain human diseases, particularly muscle, tendon, joint and ligament injuries. Musculoskeletal injuries or diseases are very common in these large animals, and damaged cartilage, ligaments and tendons are poorly healed not only in horses but also in other species. Although autologous mesenchymal stem cell (MSC) transplantation has been shown to improve the early healing response of equine articular cartilage lesions, it does not enhance long-term tissue repair.
iPSCs hold great promise for future regenerative therapies for diseases that are incurable today. Scientists have proposed that the use of iPSCs could avoid the drawbacks of MSC transplantation therapies because these cells can not only be expanded in vitro in large numbers, but can also be directed to differentiate into various specific cell types. It has been proposed that equine iPSCs can be reprogrammed from a variety of equine somatic cells and be differentiated into multiple cell and tissue types for disease modeling including myotubes, neurons, osteoblasts, and tendons. These reprogrammed iPSCs showed the direct differentiation capacity required for musculoskeletal injury repair and have encouraging therapeutic potential in equine injury disease models.
Fig.1 In vitro characterization of equine iPSCs. (Lee, 2016)
Our Services
With a best-in-class cell reprogramming platform and a highly specialized research team, Creative Bioarray offers clients a wide range of services for reprogramming equine somatic cells into iPSCs. The services we offer include, but are not limited to:
- Generating iPS cell lines from equine fibroblasts using a combination of PB transposon and tetracycline induction systems.
- Generating equine iPSCs by transduction of equine isogenic myogenic mesoangioblasts (MAB) and chondrogenic mesenchymal stem cells (MSC) with retroviral vectors carrying human reprogramming factors.
- Generating iPSCs from equine adipose-derived stem cells using a TetO lentiviral vector carrying the defined TFs.
- In vivo and in vitro characterization of equine iPS cell lines.
Applications
- Development of treatment strategies for musculoskeletal diseases
- Development of preclinical models for human applications
- Resume research on equine species currently threatened or recently extinct
- Study on production of genetically improved organisms
Advantages of Our Services
- Industry-leading scientific experts
- Flexible and efficient custom solutions
- Assistance and support throughout the entire process
Creative Bioarray has made great progress in the generation of iPSCs in domestic animals. We provide efficient technical support to help customers generate stable equine iPSCs from fetal, foal and adult equine fibroblasts and other cell types. If you need technical support in this field, please contact us.
Reference
- Lee, E.M.; et al. Generation of equine-induced pluripotent stem cells and analysis of their therapeutic potential for muscle injuries. Cell transplantation. 2016, 25(11): 2003-2016.