With extensive service experience in the field of cellular reprogramming, Creative Bioarray has actively explored strategies to assess the function of reprogrammed cells in vivo to help clients accelerate the translation of reprogramming technologies into future therapies. We are well-positioned to provide you with professional and efficient scientific solutions to advance your research.
Background
The advent of cell reprogramming technologies has accelerated the development of human disease research, drug discovery and regenerative medicine. While the original reprogramming involved reprogramming somatic cells to pluripotency through a combinatorial code, a "second generation" cell reprogramming is the direct reprogramming of one somatic cell to another type of cell through lineage-restricted TFs and microRNAs, leading to global changes in the epigenetic landscape that drive cell fate decisions. Currently, this technology is widely used to transform resident support cells in damaged organs into the desired cell type to regenerate lost tissue in situ. In vivo functional assessment of these transformed cells has been helpful in advancing the use of this technology in the clinic.
For the analysis of the function of converted cells, assessing their function in vivo is a very rigorous test. The ability of the converted cells to survive and integrate into the in vivo environment is usually first assessed in the functional analysis. Several studies have reported this assay on several murine lineages induced by lineage reprogramming, showing that only a few induced human cell types showed the ability to survive and integrate into the in vivo environment, such as hepatocytes, limbal stem cells (LSCs), neural crest cells, pluripotent hematopoietic progenitors, and endothelial cells.
Our Technique Support
To meet the diverse scientific needs of our clients in the field of cellular reprogramming, we focus on the in vivo reprogramming paradigm of regenerative medicine to develop methods for the in vivo functional assessment of reprogrammed cells. We further test the in vivo function of transformed cells primarily through the use of animal disease models. For example, we evaluated the function of mouse fibroblast induced thymic epithelial cells (TEC), which involves observing whether the induced TECs transplantation leads to the formation of a complete, well-organized and functional thymus in vivo to support the development of T cells in athymic nude mice. We provide customers with these functional measurements as an important reference for evaluating the functional maturity of transformed cells.
What Needs Customer Attention
- Possible positive results due to re-proliferation of residual target cells.
- Possible positive results due to instructive effects that result from other cell types.
- Possible role of xenogeneic rejection and interspecies differences in the transplantation process of transformed human cells.
Advantages of Us
- Advanced experimental platform
- Fully developed and improved analytical methods
- The most competitive prices
- Detailed after-sales service
Creative Bioarray is committed to providing customers with efficient functional evaluation services for converted cells in vivo. Our comprehensive technical support in the field of cell reprogramming will accelerate your research. If you need our service, please contact us for more details.
Reference
- Xu, J.; et al. Direct lineage reprogramming: strategies, mechanisms, and applications. Cell stem cell. 2015, 16(2): 119-134.