To meet the broad scientific needs of our customers in the field of cell reprogramming, Creative Bioarray has developed multiple strategies to improve cell reprogramming efficiency through biochemical cues including extracellular matrix (ECM) components, soluble factors and small molecules. We are confident that we can provide you with the necessary technical support to accelerate your research in the field of cell reprogramming.
Background
iPSC reprogramming and direct transformation are two promising methods to manipulate cell fate. The role of chemical methods and biomaterials in cell reprogramming has been extensively investigated in a large number of studies. In addition to the physical microenvironment, soluble chemical factors from the microenvironment have been shown to strongly influence the cell reprogramming process.
Biochemical cues such as ECM, growth factors, small molecules and other signaling molecules are not only involved in regulating the fate of stem cells, but also play a regulatory role in the cell reprogramming process. Small molecules and proteins can be mixed or bound to 2D culture substrates and 3D scaffolds as ECM molecules, thus having an impact on the cell fate. Notably, such biochemical stimuli can be conferred and controlled by defined delivery systems or combined with biophysical cues to maximize efficient cell reprogramming, especially in situ reprogramming.
Fig.1 Various biochemical and biophysical cues provided by matrix to regulate the native cell response. (Xiao, 2017)
Our Strategies
We have developed several strategies to improve the efficiency of cell reprogramming through biochemical regulation. Our strategies include but are not limited to:
- By using custom-engineered materials with immobilized biosignals to enhance cellular reprogramming. For example, modifying PEG hydrogels with RGD peptides and high concentrations of laminin to modulate specific cellular responses, thus improving the efficiency of fibroblast reprogramming to cardiomyocyte-like cells.
- Utilizing small molecules and growth factors to enhance or induce iPSC reprogramming or direct reprogramming to a variety of cell types including neurons, pancreatic beta-like cells, cardiomyocytes, and hepatocytes.
Applications
The development of more efficient methods to facilitate cellular reprogramming has a wide range of applications:
- Providing new insights into the study of stem cell biology.
- Provides a powerful platform for disease modeling.
- Offering a promising future for personalized medicine.
Our Advantages
- Innovative research team
- Industry-leading cell reprogramming technology services
- Integrated full process solutions
Creative Bioarray has a world-class cell reprogramming technology platform to provide high-quality reprogramming services for institutions and enterprises engaged in life science research. We have successfully helped our customers improve cell reprogramming efficiency by applying a variety of biochemical regulations. If you are interested in our services, please contact us for more details.
Reference
- Xiao, Y.; et al. Biochemical and biophysical cues in matrix design for chronic and diabetic wound treatment. Tissue Engineering Part B: Reviews. 2017, 23(1): 9-26.