Three-dimensional (3D) microenvironments can provide bionic systems to guide the fate of cells. To help our customers improve cell reprogramming efficiency, Creative Bioarray focuses on biomaterial-mediated and self-assembled cellular 3D microenvironments for use as powerful platforms to regulate cell reprogramming. Our expert technical support will help our customers to exploit the clinical potential of reprogramming technologies to a greater extent.
Background
Cell reprogramming techniques have become one of the greatest achievements in the development of cell therapy. However, the efficiencies of current reprogramming approaches for generating iPSCs are still low. Therefore, many research groups have improved reprogramming efficiency by modifying reprogramming factors or developing new methods. A variety of 2D cell culture conditions involving various biochemical cues such as various viral or RNA vectors containing multiple reprogramming factors, and protein or small molecule supplements have been applied. However, the impact of 3D microenvironments involving biophysical cues on cell reprogramming has been less reported.
3D biomaterial-based microenvironments enable robust spatiotemporal control of biochemical and biophysical cues to direct cell fate in vivo and in vitro. It has been reported that the efficiency of mouse and human iPSC reprogramming can be improved in 3D PEG hydrogels by adjusting parameters such as degradability, stiffness and biochemical composition. This 3D approach leads to much higher reprogramming efficiency compared to 2D environment due to the accelerated MET process and increased epigenetic remodeling.
Fig.1 miR combo mediated induction of αMHC expression is enhanced by 3D culture environment. (Li, 2016)
Scientific Services
The scientific services we provide to our clients include but are not limited to:
- We have developed electrospun fiber scaffolds as bionic microenvironments for biomedical applications to facilitate in situ stem cell neuronal reprogramming, leading to neural network formation and brain implantation.
- We have developed an automated microfluidic platform to improve human iPSC reprogramming efficiency by limiting the microenvironment to manipulate the concentration and sequential delivery of reprogramming factors and small molecules.
- We have developed a tissue-engineered 3D hydrogel environment to improve the efficiency of direct reprogramming, such as direct reprogramming of fibroblasts into cardiomyocytes.
Applications
- Improvement of reprogramming efficiency of human iPSC
- Disease modeling, toxicology research, and regenerative medicine
- Personalized drug screening
Our Advantages
- Innovative and integrity
- Safety and reliability
- Extensive experience in cell reprogramming services
- Customer-focused service
In order to provide high-quality cell reprogramming services to our clients, Creative Bioarray is committed to improving the efficiency of cell reprogramming by providing optimal 3D microenvironments for cells. Equipped with a state-of-the-art experimental platform and a dedicated research team, we are capable of providing the best solution for your project. Please contact us for technical support.
Reference
- Li, Y. et al. Tissue-engineered 3-dimensional (3D) microenvironment enhances the direct reprogramming of fibroblasts into cardiomyocytes by microRNAs. Scientific reports. 2016, 6(1): 1-11.